This course will focus on the latest state-of-the-art sequencing platform technology and novel library preparation protocols. We will start with experimental design, focusing on the hands-on library preparation (including best practices, standards, and potential pitfalls), concluding with sequencing of libraries, bioinformatic evaluation & best practice. For this purpose we will use, for the first time ever in the settings of the practical course, the cutting-edge sequencing platform AVITI which was developed by Element Biosciences and is currently entering the next-gen sequencing (NGS) field. An evaluation of the technology in conjunction with the newly developed library preparation reagents by New England Biolabs (NEB) will be important for the scientific community and course participants to assist their decision if to adopt the new sequencing technology. The course will cover a complete workflow, such as library preparation and sequencing. The course participants will also learn about its tips and tricks and will also use bioinformatics tools required for data processing, quality control and initial data analysis. Beside the practical exercises, lectures and discussion sessions, the course will offer the opportunity to learn more about transferring the knowledge gained during the course to the home institute of the participants as well as the implementation of this new technology into the environment of genomics core facilities. 

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Genomics Molecular Diagnostic Techniques

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